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Image Search Results
Journal: Cellular and Molecular Gastroenterology and Hepatology
Article Title: Ferroportin Expression in Adipocytes Does Not Contribute to Iron Homeostasis or Metabolic Responses to a High Calorie Diet
doi: 10.1016/j.jcmgh.2018.01.005
Figure Lengend Snippet: Quantitative Reverse-Transcription PCR Primer Sequences (5’ to 3’)
Article Snippet: A 1:10,000 dilution of primary
Techniques: Reverse Transcription
Journal: Cellular and Molecular Gastroenterology and Hepatology
Article Title: Ferroportin Expression in Adipocytes Does Not Contribute to Iron Homeostasis or Metabolic Responses to a High Calorie Diet
doi: 10.1016/j.jcmgh.2018.01.005
Figure Lengend Snippet: Adipokine expression is unchanged in FKO mice. ( A–D ) Relative mRNA expression of the adipocyte fraction of epididymal fad pads for non–insulin-stimulated animals. ( A ) Adiponectin mRNA. Diet and genotype effects were both NS (2-way ANOVA). ( B ) Leptin mRNA. The fast food diet was associated with increased Leptin mRNA ( P = .03, 2-way ANOVA). ( C ) Resistin mRNA. Diet and genotype effects were both NS (2-way ANOVA). ( D ) RBP-4 mRNA. The fast food diet was associated with decreased RBP-4 mRNA ( P = .01, 2-way ANOVA). ( E ) Immunoblotting densitometry of total serum adiponectin (non–insulin-stimulated only is represented here). Diet and genotype effects were both NS (2-way ANOVA; n = 4–6 per group). ( F ) Representative immunoblots of serum adiponectin (presented blot includes both non–insulin- and insulin-stimulated animals as indicated. I, insulin; S, saline (vehicle).
Article Snippet: A 1:10,000 dilution of primary
Techniques: Expressing, Western Blot, Saline
Journal: Cellular and Molecular Gastroenterology and Hepatology
Article Title: Ferroportin Expression in Adipocytes Does Not Contribute to Iron Homeostasis or Metabolic Responses to a High Calorie Diet
doi: 10.1016/j.jcmgh.2018.01.005
Figure Lengend Snippet: Adipokine expression is unchanged in insulin-stimulated FKO mice. ( A–D ) Relative mRNA expression of the adipocyte fraction of epididymal fad pads (insulin-stimulated state). ( A ) Adiponectin. Adiponectin mRNA was reduced by the fast food diet ( P = .0003, 2-way ANOVA). Genotype effect was NS. ( B ) Leptin. Leptin mRNA was increased by the fast food diet ( P = .01, 2-way ANOVA). Genotype effect was NS. ( C ) Resistin. Resistin mRNA was reduced by the fast food diet ( P = .01, 2-way ANOVA). Genotype effect was NS. ( D ) RBP-4. There was a significant interaction between diet and genotype ( P = .049). Post hoc analysis found RBP-4 mRNA was reduced by the fast food diet among FKO animals ( P = .0008, Sidak’s multiple comparisons test). In flox mice the results were NS (n = 4–6 per group).
Article Snippet: A 1:10,000 dilution of primary
Techniques: Expressing